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Spectroscopic methods - A

Class at Faculty of Science |
MC260P128A

Syllabus

1. Vibrational spectroscopy

Introduction to vibrational spectroscopy, infrared spectroscopy (NIR, MIR, FAR), Raman scattering, resonance Raman scattering, selection rules, molecular vibrations, vibrational bands assignments, characteristic vibrations, surface-enhanced Raman scattering (preparation of plasmonic metal nanostructures), applications 2. Electronic spectroscopy

Introduction to electronic spectroscopy, absorption and emission electronic transitions, fluorescence spectroscopy, Jablonski diagram, Franck-Condon principle, radiation and radiationless deactivation of electronic excited states, Förster resonance energy transfer, static and dynamic fluorescence quenching, relaxation of the solvent cage, fluorescence anisotropy. Application of fluorescent probes for studies of colloidal systems and biopolymers. 3. NMR spectroscopy

Principles of magnetic resonance, chemical shift, J-coupling, interpretation of 1D proton and carbon NMR spectra, APT and DEPT experiments, introduction to 2D NMR spectroscopy, COSY, HSQC and HMBC experiments and their interpretation

Annotation

Introduction to spectroscopic methods. The course combines lectures comprising both theoretical principles of the methods and their instrumentation with practical courses.