Syllabus
1. Fluorescence spectroscopy and microscopy for biology and medicine
2. Absorption, Excitation and Emission Spectra, Quantum Yield
3. Fluorescence Lifetimes
4. Quenching of Fluorescence
5. Fluorescence Polarization
6. Effects of fluorophore's environment on its spectra
7. Förster Energy Transfer, Excimer Fluorescence, Fluorescent Proteins
8. Fluorescence microscopy I, Basic concepts of optical microscopy
9. Fluorescence microscopy II, Advanced approaches
10. Fluorescence microscopy III, Fluorescence correlation spectroscopy (FCS)
11. Photodynamic Therapy Moreover, all lectures are available on the internet: http://www.jh-inst.cas.cz/~fluorescence/lectures.html
Annotation
The course gives an introduction to the principles of fluorescence and highlights relevant applications mainly in biology. The course is held every week, always two hours.
The lecturer prefers to teach that subject in English. However, for the case the students wish that the course is held in Czech language, it will be held in Czech language.