Syllabus
Basic principles of pulse Fourier transform NMR spectroscopy, magnetization, pulse angle, vector model, free induction decay (FID). Fourier transformation.
Pulse sequencies of simple one-dimensional spectra (inversion recovery, spin-echo, attached proton test). Broad-band decoupling, off-resonance decoupling, gated decoupling.
Effect of chemical exchange. Nuclear Overhauser effect.
Principles of two-dimensional spectroscopy, polarization transfer. Homonuclear correlated spectra (H,H-COSY, LR-COSY, TOCSY, NOESY, ROESY, EXSY). 2D J-resolved spectra.
Heteronuclear H,C-correlated spectra, inversion techniques (HSQC, HMBC). Connectivity of carbon atoms (2D-INADEQUATE).
Utilization of 2D spectra for structural analysis. Analysis of high-resolution NMR spectra.
NMR spectra of other nuclei.
Annotation
The scope of this course is to provide students with understanding of basic principles of pulse Fourier transform NMR spectroscopy and two-dimensional techniques (2D NMR).
Simple one-dimensional pulse sequences (inversion recovery, APT, spin-echo experiments). Nuclear Overhauser effect. Principles of two-dimensional spectroscopy, polarization transfer, homonuclear correlated spectra (COSY), J-resolved spectra, heteronuclear correlations H,C, inversion techniques. Dynamic processes studied by NMR spectroscopy. Basics of solid-state NMR spectroscopy.