Basic principles of pulse Fourier transform NMR spectroscopy, magnetization, pulse angle, vector model, free induction decay (FID). Fourier transformation.
Pulse sequencies of simple one-dimensional spectra (inversion recovery, spin-echo, attached proton test). Broad-band decoupling, off-resonance decoupling, gated decoupling.
Effect of chemical exchange. Nuclear Overhauser effect.
Principles of two-dimensional spectroscopy, polarization transfer. Homonuclear correlated spectra (H,H-COSY, LR-COSY, TOCSY, NOESY, ROESY, EXSY). 2D J-resolved spectra.
Heteronuclear H,C-correlated spectra, inversion techniques (HSQC, HMBC). Connectivity of carbon atoms (2D-INADEQUATE).
Utilization of 2D spectra for structural analysis. Analysis of high-resolution NMR spectra.
NMR spectra of other nuclei.
The scope of this course is to provide students with understanding of basic principles of pulse Fourier transform NMR spectroscopy and two-dimensional techniques (2D NMR).
Simple one-dimensional pulse sequences (inversion recovery, APT, spin-echo experiments). Nuclear Overhauser effect. Principles of two-dimensional spectroscopy, polarization transfer, homonuclear correlated spectra (COSY), J-resolved spectra, heteronuclear correlations H,C, inversion techniques. Dynamic processes studied by NMR spectroscopy. Basics of solid-state NMR spectroscopy.