Syllabus
Basic concepts, resolution of optical microscope
Overview of imaging methods and their principles
Light field, dark field, phase contrast, anoptral microscopy
Polarized light microscopy, Nomarsky method, Hoffman modulation contrast
Fluorescence microscopy
Videomicroscopy and computer image processing
Confocal microscope, near-field microscope
Special microscopic techniques, FRAP, microspectrofluorimetry
Comparison of microscopy with other optical methods of studying cell morphology
Current cytometry
Biomolecular imaging techniques
Raman and IR microscopy
TERS and SERS imaging techniques
Near field optical scanning microscopy (SNOM)
Atomic Force Microscopy (AFM)
Mass spectroscopy imaging methods (nano-SIMS)
Acoustic imaging methods (ultrasound, sonar)
Optical coherence tomography (OCT)
Interferometric scattering microscopy (iSCAT)
Positron emission tomography (PET, HRRT PET)
Methods of fluorescence microscopy with high spatial resolution
Annotation
Dielectric properties of organic compounds. Complex permittivity, mechanisms of polarization, theory of the static permittivity.
Interpretation of the frequency-dependent measurements (Debye's equations), temperature dependencies of the relaxation times. Spectrometers for dielectric measurements, selected applications of the dielectric measurements.
Transport of the ions through biological membrane. Principles of optical microscopy.
Basic concepts: resolving power of the optical microscope. Overview of the methods and their principles.
Light field, dark field, phase contrast, anoptrale microscopy, polarized-light microscopy, Nomarsky's method, Hoffman's modulation contrast, fluorescence microscopy. Videomicroscopy and computer-assisted treatment of image.
Confocal microscope, near-field microscope. Special techniques: FRAP, microspectrofluorimetry.
Confrontation of the microscopy with the other optical methods used for study of the cell morphology: light scattering, flow-cytometry.