Syllabus
Sources, detectors and spectrum analyzers in optical spectroscopy
Interaction of optical radiation with an isolated molecule. Dipole approximation
Selection rules for electronic, vibrational and rotational optical transitions.
Methods and applications of electron absorption spectroscopy. Excitation and sounding method.
Use of polarized radiation and its analysis in optical spectroscopy. Linear and circular dichroism.
Methods and applications of vibrational absorption spectroscopy.
Methods of elastic, dynamic and Brillouin scattering and their use.
Raman scattering, methods of measurement and use.
Principles and basic concepts of luminescence (types of luminescence, Jablonsky diagram, kinetics, quantum yield, lifetimes, Franck-Condon principle).
Influence of intermolecular interactions on luminescence parameters (influence of environment, resonant energy transfer, emission quenching).
Emission anisotropy
Measurement of stationary and time-resolved luminescence.
Phosphorescence, single-molecular spectroscopy. Influence of interaction with the environment on the width of the spectral line.
Nuclear magnetic resonance: principle, experimental setup, excitation and signal detection, basic pulse sequences.
High resolution NMR of organic substances in liquids: interpretation of spectra.
Electron paramagnetic resonance: principle, experimental setup, application.
Scattering and diffraction of X-rays, electrons and neutrons.
Principles of basic diffraction methods. Symmetry and structure of crystals and their determination from the diffraction pattern.
Annotation
Basics of optical spectroscopy, magnetic resonance and rentgen difraction methods used in biophysics and chemical physics.