Syllabus
Primary, secondary, tertiary and quaternary structure of proteins. Protein motives and domains.
Structure and function of important types of proteins (e.g. proteins bind DNA or nucleotides, viral or membrane proteins, etc.). Biochemical methods used in study of proteins.
Mass spectroscopy of proteins. Usage of circular dichroism, fluorescence spectroscopy, UV-VIS spectroscopy, dynamic light scattering spectroscopy, Raman nonrezonance and resonance spectroscopy, infrared spectroscopy and vibrational circular dichroism in study of proteins.
Computer databases useful in study of proteins. Computer modeling of proteins. Assignment of protein structure by means of NMR and X-ray analysis.
Annotation
The lecture brings in students to the world of proteins and it introduces the basic techniques, theoretical and experimental, used in study of proteins. Emphasis on practical usage of these techniques in protein research will be made.
The lecture is useful for students of biophysics, chemical physics, biochemistry and molecular biology.