Thematic areas of lectures

1. Introduction to phytochemical methods.

2. Methods of extraction and isolation

3. Chromatographic methods (TLC, prep. TLC, HPLC, HPLC/ MS, GC, GC/MS ) I

4. Chromatographic methods (TLC, prep. TLC, HPLC, HPLC/ MS, GC, GC/MS ) II

5. Spectroscopic methods for identification of secondary metabolites (IR, NMR, MS)

6. Overwiev of secondary metabolites

Lectures are held every odd Wednesday 8:50 – 10:20 a.m. in the seminary room in the Garden of medicinal plants.

Practical classes

Preparation of capillary, Pasteur pipete, pen for preparative thin layer chromatography

Thin layer chromatography: identification of unknown compound

Preparative thin layer chromatography

Preparation of alkaloid extract from plant

Flash chromatogryphy

GC/MS analysis of alkaloid extract and identification of alkaloids

GC-FID identification of galanthamine, lycorine and haemanthamine in extracts

Preparative HPLC+ HPLC analysis of herbal tea