Lectures Microbiology 1 (2nd year, Summer Term)

Lectures in 2 hour units 30 hours of lectures/term
Week Lecturer Theme

Kolářová: Introduction to Medical Microbiology, Basic Disciplines. Role in Health Care System. Characteristics of microorganisms- bacteria, viruses, fungi and parasites
Pavlik: Identification Techniques for Microbes
Paetříčková: Morphology and Physiology of Bacteria, Bacterial Genetics
Holada: Disinfection and Sterilisation Technics, Methods of Sterility Control
Pavlik: Interaction of bacteria and the host - Pathogenicity and Virulence,
Adámková: Antimicrobial agents and antimicrobial therapy.
Pavlík: Review of Medical Bacteriology. Gram-positive bacteria
Pavlík: Gram-negative Bacteria, Spirochets and Mycoplasma
Pavlík: Mycobacteria, Chlamydiae, Rickettsiae
Mělková: Viruses. Taxonomy. pathogenesis and intracellular parasitism in the host cell
Mělková: Selected DNA Viruses
Holada Prions and prionic infections
Mělková: Selected RNA Viruses
Skořepová: Medical Mycology
Chánová: Parasitology

Seminars (Attendance Compulsory, within the lectures, indicated in the Education Plan):

Diagnostic Techniques in Medical Microbiology (3 hours)
Rational antimicrobial therapy (3 hours)

Practicals (one week, total 24 hours):

Microscopy in medical bacteriology, mycology and parasitology. Native, fixed and stained slide. Staining techniques (Gram. Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Bacterial culture media, cultures and macroscopic morphology. Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection. Antigen detection, Typing. Antibiotic susceptibility tests (diffusion/disc tests, E-tests, MIC, MBC-testing) Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot). Genome detection techniques ? hybridisation, PCR. Horizontál electrophoresis, real-time PCR.
Urine culture and interpreting of the test. Yeast culture (Sabouraud) and GT-test.
Sample collection techniques and Kits, documentation, transport requirements.
Disinfection and Sterilisation techniques and Sterility control.
Blood cultures ? proper taking of samples, culture systéme icl. BacTec, BactAlert.
Anearobic Culture. Anaerostat.