Syllabus:

· Block 1: Historical overview and introduction (Martin Sztacho)
- Evolution of the cell nucleus
- Nuclear parameters, size, number, nr. of chromosomes
- Nuclear structure, envelope, chromatin, nucleoli
- Function, genetic information, principles of compartmentalization
- Nuclear division

· Block 2: Nuclear periphery (Peter Hoboth)
- Nuclear lamina
- Methods to measure genome organization – ChiP, proximity labelling, Hi-C
- Nuclear pore complex
- Nuclear pore complex in transcriptional regulation
- Cellular functions of inner nuclear membrane proteins

· Block 3: Principles of organization (Martin Sztacho)
- Mechanical forces in nuclear organization
- Liquid-liquid phase separation in chromatin
- Stochastic genome and its role in gene expression
- Properties and function of actin and actin filaments inside nucleus

· Block 4: Chromatin (Martin Sztacho)
- Physical nature of chromatin
- Chromatin folding and nuclear organization
- 3D chromatin modeling
- Transcription factor dynamics
- RNA in shaping the nuclear organization
- Nuclear compartments

· Block 5: Nuclear processes (Peter Hoboth)
- Space and time on the functional genome output
- DNA replication timing
- Imaging the organization of RNA processing

· Block 6: Pathophysiological processes (Peter Hoboth)
- Viruses in the nucleus
- Laminopathies
- Shaping genome during development
- Epigenetic reprograming in development
- Dynamics of X chromosome inactivation
- Organization of pluripotent genome