Microbiology exercises for general medicine I. (summer semester)

1) Introduction - safety, classification of microbes, characterization of main groups of microbes

2) Dizinfection and sterilization - fyzical and chemical methods and agents, indication and quality control, laboratory equipment and its application

3) Cultivation I. - specimens collection, processing and storage, culture media characteristics

4) Cultivation II. - seeding of culture media, characteristics of microbial growth

5) Microscopy I. - Gram stain, bacterial morphology

6) Microscopy II. - Acid-fast stain, negative stain, slides of clinical specimens, host cells identification

7) Cultivation III. - Biochemical and additional tests

8) Cultivation IV. - Antibiotic susceptibility testing

9) Immunological methods I.- terms definition, polyclonal and monoclonal antibodies charteristics, production and application,

10) Immunological methods II. - Principles of direct and indirect immunological methods

11) Immunological methods III. - Main assays, its characteristics, application and limits in diagnostics

12) Identification of medically important microbes - main groups of microbes in cultures and microscopy slides