Gastric submucosal microdialysis in detection of rat stomach ischemia - a comparison of the 3H2O efflux technique with metabolic monitoring
Abstract
Comparison of microdialysis approaches to measure blood flow in the stomach is lacking. We compared 3H2O efflux technique with biochemical monitoring during temporary celiac artery occlusion in rats.
Probes were implanted in the gastric submucosa and perfused with 3H2O; samples were analyzed for β-activity, glucose, lactate, pyruvate and glycerol. Gastric mucosa and plasma were subjected to morphometry and analysis of myeloperoxidase, thiols and lactatdehydrogenase.
The most dramatic responses were observed in lactate/pyruvate and lactate/glucose (%) ratios; the change in 3H2O efflux was less pronounced. 3H2O efflux correlated best with lactate/glucose ratio. A correlation was also found between plasma lactatdehydrogenase and relative glycerol release.
Myeloperoxidase, lactatdehydrogenase and histology score were increased by ischemia-reperfusion, macroscopy and plasma thiols remained unchanged. Microdialysis metabolic monitoring is superior to the 3H2O efflux technique.