Abstract
Objective: To compare 3H2O efflux technique with biochemical monitoring during temporary celiac artery occlusion in rats under general anesthesia. Material and methods: Animals were divided into two groups – ischemia-reperfusion (n=9) and sham-operated (n=7).
Microdialysis probes were implanted in the gastric submucosa and perfused with a flow indicator 3H2O - containing solution. The samples were analysed for 3H β-activity and glucose, lactate, pyruvate and glycerol concentrations.
Results: The most dramatic responses to ischemia were observed in the relative changes of metabolic ratios lactate/pyruvate and lactate/glucose, the changes in flow indicator 3H2O efflux and glycerol were less pronounced. The efflux technique correlated best with lactate/glucose ratio and glucose alone.
Conclusion: Microdialysis is useful in monitoring gastric ischemia, metabolic monitoring being superior to the efflux technique.