Crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein FrpD from Neisseria meningitidis

Abstract

Fe-regulated protein D (FrpD) is a Neisseria meningitidis outer membrane lipoprotein that may be involved in the anchoring of the secreted repeat in toxins (RTX) protein FrpC to the outer bacterial membrane. However, the function and biological roles of the FrpD and FrpC proteins remain unknown.

Native and selenomethionine-substituted variants of recombinant FrpD43–271 protein were crystallized using the sitting-drop vapour-diffusion method.

Keywords

• crystallization
• crystallographic characterization
• iron-regulated proteins
• lipoprotein FrpD
• Neisseria meningitidis