The binding affinity of carcinogenic N-nitrosodimethylamine and N-nitrosomethylaniline to cytochromes P450 2B4, 2E1 and 3A6 does not dictate the rate of their enzymatic N- demethylation.

Abstract

The interaction of carcinogenic N-nitrosodimethylamine (NDMA) and N-nitrosomethylaniline (NMA) with cytochromes P450 (CYP), CYP2B4, CYP2E1 and CYP3A6, and their metabolism by these enzymes reconstituted with NADPH-CYP reductase in liposomes were studied in this work.

Keywords

• liver-microsomes
• active-site
• in-vitro
• nitrosamine metabolism
• p-450 reductase
• cell-line
• dna
• denitrosation
• adducts
• model