Abstract
The aim of this study was to investigate the effect of high-protein and high-glutamine diet on bone metabolism in rats for 16 weeks. Male Wistar rats were divided into 3 groups of 6 animals. 1. group (KO): standard laboratory diet (SLD), 2. group (HPD): high-protein diet with casein, 3. group (GLN): glutamine-enriched diet.
We assayed marker of bone resorption-C-terminal telopetide of collagen I (ICTP) and markers of bone formation- osteocalcin (OC) and N-terminal propeptide of procollagen I (PINP) by ELISA. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry (DXA).
Concentration of ICTP significantly (p<0,05) decreased by 33 % in GLN vs. KO.
Concentrations of OC, PINP and BMD in tail were decreased, BMD in femur were increased in GLN vs. KO, but there were no statistical significance.
GLN and HPD groups had decreased markers of bone turnover which contributed to decreasing in tail BMD.