Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance study
2010
Abstract
We investigated the simultaneous effect of monovalent and divalent cations on hybridization of DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrated that divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium.
This trend is opposite to that established for oligonucleotides in a solution. Moreover, the selected saline composition of buffer improved the discrimination of complementary and point mismatched DNA targets.