An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part II: Oxidative damage to DNA
Abstract
The aim of our study was to investigate the ability of organic extracts of size segregated aerosol particles (EOM; three fractions) to induce oxidative damage to DNA in an in vitro acellular system CT DNA with/without S9 metabolic activation. PM was collected in the Czech Republic at 4 places with different air pollution levels.
Levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) tended to increase with decreasing sizes of PM. S9 metabolic activation increased the oxidative capacity of PM.
We observed a significant positive association between concentrations of c-PAHs bound to PM and levels of 8-oxodG/105 dG per 1000 m3 of air after metabolic activation of EOM samples (R = 0.695, p <0.05). The correlation was weaker and nonsignificant for samples without metabolic activation (R = 0.523, p = 0.08).
We showed that organic extracts of PM were able to induce oxidative damage to DNA in vitro; this ability was increased after S9 metabolic activation of EOM and with decreasing sizes of PM.