Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape

Abstract

Twelve doubled haploid (DH) winter oilseed rape plants with altered levels of proline and/or freezing tolerance were obtained by in vitro selection for resistance to trans-4-hydroxy-l-proline in five segregating microspore populations. When data from all examined materials were combined, a weak correlation between proline content and freezing tolerance was observed.

Keywords

• Freezing tolerance
• proline
• Brassica napus
• microspore cultures
• in vitro selection