Abstract
Previously characterized Bacillus subtilis mutant, defective in growth and osmoadaptation under limited K+ concentration. In this mutant the yxkO gene coding putative ribokinase is disrupted.
This mutant reveals a pleiotropic phenotype compared to WT. It differs in osmosensitivity,cell shape, motility under the stress condition contrary to WT (Bacillus subtilis168).
In microarrays study increase of transcription after osmotic, heat and ethanol shock was detected and was predicted that its belong to sigma B operon. We have used 2DE approach to analyzed cytoplasmic proteome of the wt and mutant under osmotic and ethanol shock.
Several differences in protein expression pattern were identified. We have found that knock out of yxkO gene influence expression of flagellin, malate dehydrogenase, phosphoglycerate kinase, PTS enzyme I, glutamine synthetase, GroeL, F1/Fo ATPase subunit, fructosolysine-6-phosphate-deglycase and enzymes for branched amino acids synthesis.