Abstract
Tetracycline resistance (TET-r) belongs to the widespread antibiotic-resistance in environment, and represents important global anthropogenic loading for the environment. We screened TET-r genes in selected 12 TET-r Gram positive and Gram negative bacterial strains isolated from both agricultural soil with a long history of fertilization containing TET residues. 22 primers were tested, designed for effluxes, for ribosomal protection proteins and inactivators.
The tet(D)-specific primer yielded PCR products in three isolates of Stenotophomonas sp. and five isolates of Chryseobacterium sp. The fragment sequence revealed gene coding sodium/solute symporter-histidine kinase hybrid protein specific for Stenotrophomonas species which was not yet described as resistant determinant.
The presence of the genes encoded the protein typical for Stenotrophomonas sp. detected also in Chryseobacterium indicated recent horizontal transfer between both evolutionary divergent genera in the investigated soils.