Methods for determination of all binding parameters in systems with simultaneous borate and cyclodextrin complexation
Abstract
Two novel methods for determination of binding constants in the systems with borate and cyclodextrin complexation were developed. The methods enable to determine all binding parameters in these systems and even the binding constants of interaction of a neutral analyte with a neutral cyclodextrin.
The first method is based on nonlinear fitting of experimental data and further evaluation of fitting parameters. The second method requires a multiple regression.
The methods provide identical results with low experimental error. Only one set of measurements is required for both methods.
Thus the binding parameters can be mutually compared. The binding parameters for neutral analytes ((R,R)-(+)-hydrobenzoin and (S,S)-(-)-hydrobenzoin) and neutral cyclodextrin (heptakis(2,6-di-O-methyl)-beta-cyclodextrin) were evaluated and the effect of individual types of interaction was revealed.