Proteomic and transcriptomic analysis of heart failure due to volume overload in a rat aorto-caval fistula model provides support for new potential therapeutic targets - monomaine oxidase A and transglutaminase 2
Abstract
Background: Chronic hemodynamic overloading leads to heart failure (HF) due to incompletely understood mechanisms. To gain deeper insight into the molecular pathophysiology of volume overload-induced HF and to identify potential markers and targets for novel therapies, we performed proteomic and mRNA expression analysis comparing myocardium from Wistar rats with HF induced by a chronic aorto-caval fistula (ACF) and sham-operated rats harvested at the advanced, decompensated stage of HF.
Methods: We analyzed control and failing myocardium employing iTRAQ labeling, twodimensional peptide separation combining peptide IEF and nano-HPLC with MALDIMS/ MS. For the transcriptomic analysis we employed Illumina RatRef-12v1 Expression BeadChip.
Results: In the proteomic analysis we identified 2030 myocardial proteins, of which 66 proteins were differentially expressed. The mRNA expression analysis identified 851 differentially expressed mRNAs.