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Determination of carbethopendecinium bromide in eye drops by capillary electrophoresis with capacitively coupled contactless conductivity detection

Publication at Faculty of Pharmacy in Hradec Králové |
2011

Abstract

Septonex was determined by capillary electrophoresis with capacitively coupled contactless conductivity detection. Optimal separation was achieved in BGE of pH 7.0 containing 30mM 2-(N-morpholino) ethanesulfonic acid, 12.5 mg/mL of 2-hydroxypropyl-beta-cyclodextrin and 20% v/v of acetonitrile.

The separation was performed at 25 degrees C in an uncoated fused silica capillary (50 mm id; total length, 60.5 cm; effective length, 50 cm) at 30 kV. Samples were injected hydrodynamically at 50 mbar for 6 s.

For quantitative analysis, L-arginine (500 mu g/mL) was used as internal standard. The LOD was 7 mu g/mL of septonex (at S/N = 3).

The run-to-run repeatability (n = 6) was characterized by the RSDs of 0.18% for the migration time and 1.96% for the analyte/internal standard peak area ratio. Accuracy tested by recovery experiments at three concentration levels gave recoveries of 100.27-104.22% with RSD <= 2.19%.

The method was applied to the assay of carbethopendecinium bromide in eye drops.