Determination of muscle relaxants pancuronium and vecuronium bromide by capillary electrophoresis with capacitively coupled contactless conductivity detection
Abstrakt
Capillary electrophoretic method for the separation of pancuronium (PM) and vecuronium (VM) utilizing capacitively coupled contactless conductivity detection was devised. The separation was carried out in fused-silica capillaries (50 mu m id, 75/45 cm).
Optimal BGE was 50 mM borate buffer of pH 9.5 containing 12.5 mg/mL of (2-hydoxypropyl)-gamma-CD. Calibration curves were linear for both pancuronium bromide (PMB) and vecuronium bromide (VMB) in the range 25-250 mu g/mL with r > 0.9968.
The limits of detection were 7 and 6 mg/mL for PMB and VMB, respectively. The accuracy tested by recovery experiment at three concentration levels of added PMB and VMB was satisfactory (95.7-102.7%, n = 3, with RSD < 2.61%).
The method was successfully applied to the assay of PMB and VMB in commercial injection solutions.