Determination of ascorbic acid with Wells-Dawson type molybdophosphate in sequential injection system
2011
Abstrakt
Two-electron reduced heteropolyblue is formed very fast at pH 3.75-4.75 in the reaction between Dawson-type molybdophosphate HPA [image omitted] and ascorbic acid (AsA) (epsilon 814=1.15x104 L center dot mol-1cm-1). Simple, fast, and direct SIA method for AsA determination was developed.
Under found optimal conditions, linear calibration curve was obtained over the range 3x10-6-3.5x10-4molL-1 AsA, and detection limit (3s) was 1x10-6molL-1. The proposed SIA method has high sample throughput of 60h-1 and small reagent consumption.
The procedure was applied to the analysis of pharmaceuticals and juices.