Pyrazinecarboxamides as Potential Elicitors of Flavonolignan and Flavonoid Production in Silybum marianum and Ononis arvensis Cultures In Vitro
2011
Abstract
The effect of new synthetic pyrazinecarboxamide derivatives on flavonolignan and flavonoid production in S. marianum and O. arvensis was investigated.Compound I 5-(2-hydroxybenzoyl)-pyrazine-2-carboxamide has shown to be an effective elicitor of flavonolignan and taxifoline production in S. marianum culture in vitro.The maximum content of silymarin complex(0.08 %) in calluss culture was induced by 168 h elicitor application of concentration of 1.159x10-4 mol/L. All 3 tested concentrations of compound II N-(2-bromo-3-methylphenyl)-5-tert-butylpyrazin-2-carboxamide increased the flavonoid production in callus culture O. arvensis.