Hydrophilic interaction liquid chromatography in the separation of a moderately lipophilic drug from its highly polar metabolites - The cardioprotectant dexrazoxane as a model case

Abstract

Hydrophilic interaction liquid chromatography (HILIC) was used to analyze dexrazoxane and its three metabolites on different stationary phases. Initial experiments were done with HPLC-UV and subsequently LC-MS were used to confirm applicability of the selected conditions.

In addition, LC-MS behavior of all analytes were studied and characterized.

Keywords

• Hydrophilic interaction liquid chromatography
• Dexrazoxane
• Bisdioxopiperazine
• Polar drug
• LC-MS