Abstrakt
This work deals with the determination of aminoglutethimide (AGT) using high performance liquid chromatography with UV spectrophotometric detection (HPLC-UV) and amperometric detection (HPLC-ED) on carbon paste electrode (CPE). AGT was used as anticancer drug, especially for the treatment of breast cancer in postmenopausal women or for the treatment of prostate cancer.
The concentration dependences of AGT were measured by HPLC-UV with detection wavelength 242 nm, by HPLC-ED with a working potencial of +1.3 V in mobile phase consisting of 1.10-2 mol l-1 phosphate buffer (pH 4) and methanol 50:50 (v/v). The following limits of detection were achieved : 3.6.10-7 mol l-1 AGT for UV spectrophotometric detection, 2.5.10-7 mol l-1 AGT for amperometric detection.
AGT was also determined in model samples of urine. It was not possible to detect AGT with HPLC-UV, because of the interferences of other compounds.
With HPLC-ED the detection limit 5.2.10-7 mol l-1 AGT was achieved.