Polarographic and voltammetric determination of genotoxic 2-aminofluoren-9-one at mercury electrodes
Abstract
Electrochemical behavior of genotoxic 2-aminofluoren-9-one (2-AFN) was investigated by DC tast polarography (DCTP) and differential pulse polarography (DPP), both at a classical dropping mercury electrode (DME), and by DC voltammetry (DCV), differential pulse voltammetry (DPV), and adsorptive stripping differential pulse voltammetry (AdSDPV), all at a miniaturized hanging mercury drop minielectrode (HMDmE), in buffered aqueous-methanolic solutions. Optimum conditions were found for the determination of 2-AFN by DCTP at DME in the concentration range from 1 x 10-6 to 1 x 10-4 mol l-1 (with a limit of quantification (LQ) of 5 x 10-7 mol l-1), by DPP at DME (from 1 x 10-7 to 1 x 10-4 mol l-1; LQ on level of 1 x 10-7 mol l-1), by DCV and DPV at HMDmE (both from 1 x 10-7 to 1 x 10-4 mol l-1; LQs on level of 2 x 10-7 and 1 x 10-7 mol l-1 for DCV and DPV, respectively), and by AdSDPV at HMDmE (from 2 x 10-9 to 1 x 10-7 mol l-1; LQ ALMOST EQUAL TO 4 x 10-9 mol l-1).
Practical applicability of the developed methods was verified on the direct determination of 2-AFN in model samples of drinking and river water in nanomolar to micromolar concentrations.