120 unrelated individuals from the population sample of the Czech Republic were genotyped using the AmpFlSTR NGM PCR amplification kit (Applied Biosystems). The population study was conducted to evaluate the usefulness of the five new STR loci (D10S1248, D22S1045, D2S441, D1S1656, and D12S391) included in the new European Standard Set and to establish the allele frequencies.