Publication at Faculty of Science |
2011
Abstract
Optimised culture conditions for the recombinant prototrophic strain Escherichia coli RE3(pSEKau2) were established to obtain biomass of epoxide hydrolase (EH). The recombinant enzyme was extracted from crude extracts using an optimised polyethylene glycol-based aqueous two-phase system (ATPS).
Eupergit C 250L carrier proved to be the most interesting in terms of partially purified EH immobilization. The immobilized EH exhibited a significantly higher thermal stability compared to the free enzyme.
Immobilization did not alter, to a large extent, the enantioselectivity and regioselectivity of the enzyme