Abstract
A mini-review on the current state in serum 25-hydroxyvitamin D measurement. The review of used routine methods for the measurement the 25-hydroxyvitamin D in serum.
The assessment of analytical quality by means of inter-laboratory comparison programs is presented. The current state of metrological reference and standardization is outlined.
The methods, based on separation, with immunochemistry methods are compared. Problem of suitable analytes as subjects of measurement.
Stability of analyte is described. There is the necessity of standardization for achieving the interlaboratory comparability and clinical utility by means of using reference material and reference method in routine practice.