Hematopoietic Stem Cells Survive Circulation Arrest and Reconstitute Hematopoiesis in Myeloablated Mice

Abstrakt

Hematopoietic stem and progenitor cells (HSPC) for bone marrow transplantation are currently obtained directly from living voluntary donors or from cord blood units. However, a suitable donor is not always found.

Because HSPC are known for their relative resistance to hypoxia, using an experimental murine model, we explored cadaveric bone marrow (BM) as their alternative source. This paper shows that HSPC survive the cessation of circulation for a considerable time and maintain their engraftment potential.

This time is significantly extended with in vitro storage compared to the cadaveric BM.

Klíčová slova

• heart-beating donors
• cadaveric organ donors
• colony-forming cells
• bone-marrow
• overnight storage
• short-term
• in-vivo
• transplantation
• spleen
• proliferation