Detection of point mutations in Kirsten ras 2 gene using Locked Nucleic Acids clamped PCR

Abstract

The aim of the study was to examine diagnostic possibilities of LNA-clamped PCR for detection of somatic mutations in the K-ras gene in 133 samples of human malignant colorectal tumors. Selective real-time PCR amplification of mutant alleles of the K-ras gene (colon 12 and 13) revealed the presence of mutations in 45 samples of the tumors.

The detection limit of the used method for K-ras mutant alleles was 0,03 ng.