LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas

Abstract

The aim of this study is to demostrate possibility of detection of Ki-ras with a synthetic nucleotide analogue, locked nucleic acid (LNA).We consider LNA-clamped real-time PCR to be a fast alternative method for specific analysis of the Ki-ras gene in colorectal carcinomas. The reliability determined in CRC tissue was comparable with the enriched PCR/RFLP and nucleotide sequencing.