Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method
2012
Abstract
Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde.
We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found.
We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45)