The paper aimed to even more increase the production of isoflavonoids in callus culture of G. tinctoria by the action of abiotic elicitors. A more suitable elicitor for production of isoflavonoids proved to be N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide, even though the absolute maximum of genistin production was achieved by a 12 h action of 5-terc-butyl-6-chloro-N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide.