Deep-UV-LEDs in photometric detection: A 255 nm LED on-capillary detector in capillary electrophoresis

Abstract

A 255 nm deep-UV-light-emitting diode (deep-UV-LED) is investigated as a novel light source for photometric detection in view of fundamental properties of UV-LEDs, in particular emission spectra and energy conversion. Its performance in oncapillary photometric detection in capillary electrophoresis (CE) is determined and the potential of deep_UV-LEDs in optical detection is discussed.

Keywords

• light-emitting diode
• absorbency detector
• effective pathlength
• separation
• anions
• CE
• linearity
• complexation
• fluorescence
• electrolyte