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Effects of amlodipine on bone metabolism in male albino Wistar rats

Publication at Faculty of Medicine in Hradec Králové |
2011

Abstract

The aim of our study was to investigate the effect of amlodipine on bone metabolism in male rats. Amlodipine (0.3 mg/100 g BW; gavage) was administered to 8 rats for 8 weeks.

Control group (n=8) received aqua pro inj.. Bone marker concentrations of CTX-I and PINP in serum, and BALP in both serum and bone homogenate were measured by ELISA.

We investigated expression of BMP-2 in tibia using Western blot, and bone mineral density was measured by DXA in lumbar and caudal vertebrae and in femoral areas. Femurs were used for biomechanical testing.

After 8 weeks of amlodipine administration there was decrease in serum levels of BALP (p=0.0009) and CTX-I (p=0.003), and level of BALP in bone homogenate (p=0.026) compared to controls. Expression of BMP-2 was increased after amlodipine administration.

Our findings suggest that amlodipine has retarding influence on bone metabolism in rats by decreasing bone turnover, which probably in consequence increases expression of BMP-2.