Abstrakt
The aim of this study was to develop and validate new methods for the determination of active compounds in topical gels. The method for the determination of nonoxinol-9 and trimecaine hydrochloride in lubricant gel by HPLC with UV detection was described.
Separation was carried out using a Discovery ZR-PBD analytical column (150 x 4.6 mm, 5 mu m), and a mobile phase composed of a mixture of triethylammonium acetate buffer (pH 9; 50 mM) and methanol (22 : 78, v/v). Determination of terpinen-4-ol in lubricant gel was achieved by GC with a flame ionization detector (FID).
An Alltech-AT-624 (0.32 mm ID x 30 m, 1.8 mu m film thickness) fused-silica capillary column and helium as the carrier gas, at a flow rate of 28 cm s(-1), were employed. The analysis time was less than 7.5 min for HPLC and 5 min for GC analyses.
Both methods were successfully applied to the analysis of pharmaceuticals.