Rapid sample preparation procedure for determination of retinol and alpha-tocopherol in human breast milk

Abstract

The liposoluble vitamins (retinol and alpha-tocopherol) concentration in human breast milk is of a cardinal knowledge especially for nutrition of prematurely born. It enables the feeding optimization of these important micronutrients for preterm infants.

The novel rapid liquid-liquid extraction procedure for human breast milk investigation was developed and validated according to FDA guidelines.

Keywords

• Human breast milk
• Retinol
• alpha-Tocopherol
• LLE
• Sample preparation
• Monolithic column