Voltammetric DNA biosensor based on a microcrystalline natural graphite-polystyrene composite transducer
Abstract
New voltammetric DNA biosensor based on a microcrystalline natural graphite-polystyrene composite film in the role of a transducer was used for the investigation of the interaction between model carcinogenic substance (2-aminofluorene; one of the most extensively studied examples of the aromatic amine class of carcinogens) and calf thymus double-stranded DNA (dsDNA). The layer of dsDNA immobilized at the electrode surface was utilized as a biocomponent responsive interface.
The biosensor was characterized regarding the detection of DNA damage (induced by direct interaction with 2-aminofluorene) using square wave voltammetric responses of the guanine and adenine moieties and cyclic voltammetric responses of the anionic redox indicator [Fe(CN)6]4-/3- present in the solution.