Raman spectroscopy is one of the techniques that is being advocated for adoption in astrobiology for the detection of extinct or extant life on planetary surfaces extraterrestrially. A discussion on the definition of the Raman spectroscopic limits of detection and the operational factors which influence the data observed is needed for the evaluation of the results.
This research paper forms a starting point for the discussion of the meaning of detection limits of biomarker molecules - solids dispersed in solid matrices - and the consequences for astrobiological research as this will have implications on the analytical protocols and spectrometer design for remote field-operated spectrometers.