"Alternative" biological material for routine analysis of nucleic acids - validation of the pre-analytical phase of DNA testing
2012
Abstract
The aim of the study is to compare properties of DNA extracts prepared using two different isolation procedures from buccal cells, urine sediment, nails, hair roots, and peripheral blood cells. Biological material was collected from 24 volunteers at median age of 31 years (range 25-54 years).
Phenol-chloroform extraction and spin microcolumn extraction method were used for DNA isolation. The highest DNA concentrations were found in extracts from peripheral blood and buccal cells; the lowest DNA concentrations were in hair extracts.
All types of the extracts had acceptable purity. Non-fragmented DNA in the extracts decreased as follows: blood - buccal cells - urine sediment - nails - hair roots.