Publication at Faculty of Science |
2013
Abstract
Chicken antibodies (IgY) are increasingly being used as a primary antibody in the Western blotting immunodetection, offering several advantages compared to the common mammalian antibodies. The presence of additional bands in the region of 45-70 kDa, vertical streaks, and spots on the membrane, caused by human keratins, made the evaluation of the developed blots hardly possible.
In this study, we describe a method how to eliminate the cross-reactivity of IgY with human keratins.