Determination of 4-Aminobiphenyl and 4-Nitrobiphenyl by HPLC with Electrochemical, UV Spectrophotometric, and Fluorescent Detection
Abstract
HPLC systems with electrochemical, UV spectrophotometric, and fluorescent detectors were used to determine genotoxic derivatives of biphenyl -4-aminobiphenyl (4-ABP) and 4-nitrobiphenyl (4-NBP). As 4-NBP cannot be determined by electrochemical and fluorescent detector directly, reduction of 4-NBP to 4-ABP (making use of a titanium(III)-based reducing agent) was investigated and optimized.
UV detection was found to be the least sensitive and fluorescent detection the most sensitive one. Limits of detection (LDs) for 4-ABP in the presence of a reducing agent were 6.0 x 10(-6) mol L-1, 2.0 x 10(-6) mol L-1, and 8.0 x 10(-8) mol L-1 for UV, electrochemical, and fluorescent detection, respectively.
For the determination of 4-NBP after its reduction to 4-ABP, the obtained LDs were 8.0 x 10(-6) mol L-1 (UV), 4.0 x 10(-6) mol L-1 (electrochemical), and 2.0 x 10(-7) mol L-1 (fluorescent detection). Moreover, it was also possible to determine 4-NBP directly using HPLC with UV detection, where the LD was 4.0 x 10(-7) mol L-1.