The Effect of Pyridinecarbothioamides on Isoflavonoid Production in Genista tinctoria Cultures in Vitro
Abstract
The effect of 2-(3-bromobenzylsulfanyl) pyridine-4-carbothioamide on isoflavonoids production in Genista tinctoria culture in vitro was tested. The highest isoflavonoid content (0.3 mg.g(-1) DW) in callus culture was found after elicitor application at a concentration of 2.96 x 10(-5) mol.L-1 after 24 h of treatment.
The maximum daidzein production (0.2 mg.g(-1) DW) was detected after 48 h of elicitor treatment at a concentration of 2.96 x 10(-3) mol.L-1 and after 24 h of elicitor treatment at a concentration of 2.96 x 10(-5) mol.L-1. Suspension culture produced the highest amount of isoflavonoids (0.3 mg.g(-1) DW) after elicitor application at concentrations of 2.96 x 10 mol.L-1 after 24 and 168 h of treatment, and 2.96 x 10(-5) mol.L-1 after 168 h of treatment.
The highest content of genistin (0.3 mg. g(-1) DW) was detected after 24 h of elicitor treatment at a concentration of 2.96 x 10(-4) mol.L-1. The maximum content of daidzein (3.80 mg.L-1) released into the suspension culture medium was found after elicitor application at a concentration of 2.96 x le mol.L-1 after 12 h of treatment.