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Optimized Protocol of Chicken Antibody (IgY) Purification Providing Electrophoretically Homogenous Preparations

Publikace na Přírodovědecká fakulta |
2013

Tento text není v aktuálním jazyce dostupný. Zobrazuje se verze "en".Abstrakt

Chicken antibodies isolated from egg yolks (IgY) are a suitable alternative to conventional antibodies from blood of experimental animals. Main advantages of IgY are: (i) larger amounts of IgY could be prepared from repeatedly laid chicken eggs, (ii) collection of eggs is a non-invasive procedure contrary to bleeding of mammals, and (iii) a better response of chicken to mammalian antigens due to the larger evolutionary distance.

Here, we report a cost- and labor-effective two-step procedure consisting of yolk extraction by tap water (8-fold dilution, freezing, and filtration) followed by a specific precipitation of IgY at pH 4 with sodium chloride in the final concentration of 8.8%. Using this procedure, the highly purified antibody (97%) was prepared.

Resulting IgY preparations are acceptable for a wide range of applications as it was prepared using only bio-compatible chemicals. The purification protocol was developed and optimized in terms of the time, materials and the necessary manipulations.