Preclinical Tests and Small Clinical Study Evaluating the Efficacy of the Therapy of 5-FU Induced Palmar-Plantar Erythrodysesthesia with 10% Uridine Ointment
Abstract
Introduction: Authors present the results from original preclinical research regarding with the protective effect of uridine on human keratinocytes treated with 5-fluorouracil (5-FU). The specificity of the uridine protective effect for 5-FU induced damage is confirmed by comparison of the cell viability treated with 5-FU and the viability of cells treated with paclitaxel.
The authors also present the results of small clinical study examining the patient satisfaction with local treatment of hand-foot syndrome by 10% uridine ointment. Methods: Viability of cell culture (HaCaT cells - spontaneously immortalized human keratinocytes) treated with 5-FU is evaluated by three different methods: NTCA test (nondestructive test of cellular activity), MTT test and RTCA (real time cell analyser).
The results were obtained by means of questionnaire. Results: Authors proved the ability of uridine to protect the HaCaT cells from the 5-FU induced damage.
This effect was specific for 5-FU, because uridine did not prevent the damage caused by paclitaxel. Only one fourth of the patients returned the questionnaire (90). 56 % (49) of these patients classified the effect of uridine ointment as mild or good improvement. 3 % (3) evaluated the effect as insufficient and the rest (38) did not answer due to the short duration of treatment.
Discussion: The results of in vitro testing and small clinical study support the meaningfulness of 5-FU induced palmar-plantar erythrodysesthesia treatment by 10% uridine ointment local application. Acknowledgement: This work was supported by grant IGA MZ ČR, NS9786-4/2008.